Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

@inproceedings{Shirato2014DetectionOM,
  title={Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)},
  author={Kazuya Shirato and Takuya Yano and Syouhei Senba and Shigehiro Akachi and Takashi Kobayashi and Takamichi Nishinaka and Tsugunori Notomi and Shutoku Matsuyama},
  booktitle={Virology Journal},
  year={2014}
}
The first documented case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) occurred in 2012, and outbreaks have continued ever since, mainly in Saudi Arabia. MERS-CoV is primarily diagnosed using a real-time RT-PCR assay, with at least two different genomic targets required for a positive diagnosis according to the case definition of The World Health Organization (WHO) as of 3 July 2013. Therefore, it is urgently necessary to develop as many specific genetic diagnostic methods as… CONTINUE READING
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