Detection of Methane Oxidizing Bacteria in Forest Soil by Monooxygenase PCR Amplification

@article{Jensen2000DetectionOM,
  title={Detection of Methane Oxidizing Bacteria in Forest Soil by Monooxygenase PCR Amplification},
  author={Sigmund Jensen and A. J. Holmes and Rolf A. Olsen and John Colin Murrell},
  journal={Microbial Ecology},
  year={2000},
  volume={39},
  pages={282-289}
}
Atmospheric methane oxidation by a spruce forest soil from Norway at 15°C was found to be maximal at a depth of ca 7 cm. Examination of the kinetics of this methane oxidation revealed an apparent K m of 403.1 nM and a V max of 2.2 nmol g-1 dry weight soil h-1. The low apparent K m suggested the presence of active methane oxidizing bacteria with a high affinity for methane. DNA was extracted from the 5–10 cm horizon, purified, and subjected to PCR amplification with primers directed toward the… CONTINUE READING