Detection of Man5GlcNAc and related free oligomannosides in the cytosol fraction of hen oviduct.

@article{Iwai1999DetectionOM,
  title={Detection of Man5GlcNAc and related free oligomannosides in the cytosol fraction of hen oviduct.},
  author={Kaoru Iwai and Tomohiro Mega and Sumihiro Hase},
  journal={Journal of biochemistry},
  year={1999},
  volume={125 1},
  pages={
          70-4
        }
}
The presence of free oligomannosides in cytosol has been demonstrated by metabolic radiolabeling, and Manalpha1-2Manalpha1-2Manalpha1-3(Manalpha1-6)++ +Manbeta1-4GlcNAc was detected as the main free oligomannoside in Chinese hamster ovary cells [Kmiécik, D., Herman, V., Stroop, C.J.M., Michalski, J.C., Mir, A.M., Labiau, O., Verbert, A., and Cacan, R. (1995) Glycobiology, 5, 483-494]. In the present paper, the isomeric structures and amounts of oligomannosides in the cytosol fraction of hen… 
Man2C1, an α-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol
TLDR
The findings in the present study demonstrate that an α-mannosidase, Man2C1, is involved in the processing of free oligosaccharides that are formed in the cytosol, and may represent the common ‘non-lysosomal’ catabolic pathway for N-glycans in animal cells, although the molecular mechanism as well as the functional importance of such processes remains to be determined.
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Analysis of FOS of the nematode Caenorhabditis elegans concluded that significant amounts of misfolded glycoproteins in C. elegans are trafficked to the Golgi and are directly or indirectly retro-translocated into the cytosol to be degraded.
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The findings from enzyme-substrate characterizations and subcellular localization studies support the suggested role for neutral alpha-mannosidase in hydrolysis of soluble cytosolic oligomannosides.
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The enzymes involved in generation and degradation of FOS in the nematode Caenorhabditis elegans are characterized and Peptide: N-glycanase (PNGase) was shown to be a unique bifunctional enzyme having both deglycosylation and protein disulfide reductase activities.
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  • Biology, Chemistry
    Biochemical and biophysical research communications
  • 1999
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This review will illustrate how the different species of N-linked and free oligomannosides either are involved or are markers of the quality control and fate of newly synthesized glycoproteins.
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The results provide an insight into a possible link between catabolism of fOSs and cell wall stress.
Free Oligosaccharides to Monitor Glycoprotein Endoplasmic Reticulum-associated Degradation in Saccharomyces cerevisiae*
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A method for the isolation of yeast free oligosaccharides (fOSs) using endo-β-1,6-glucanase digestion is reported, providing valuable information on the molecular mechanism of glycoprotein ERAD in Saccharomyces cerevisiae.
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