Detection of Maedi-Visna virus antibodies using a single fusion transmembrane-core p25 recombinant protein ELISA and a modified receiver-operating characteristic analysis to determine cut-off values.

Abstract

The core p25 and transmembrane (TM) genes of Maedi-Visna virus (MVV) were cloned individually into the pGEX-2T expression vector. Both proteins were expressed as a combined fusion protein in frame with glutathione S-transferase (GST). The purified recombinant antigens (GST-TM and GST-TM-p25) were used to develop a MVV ELISA. A preliminary assessment of the… (More)

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@article{Boshoff1997DetectionOM, title={Detection of Maedi-Visna virus antibodies using a single fusion transmembrane-core p25 recombinant protein ELISA and a modified receiver-operating characteristic analysis to determine cut-off values.}, author={Christopher H Boshoff and Baptiste K. Dungu and Roger Williams and Johan Vorster and Johannes D Conradie and Daniel W Verwoerd and Denis F York}, journal={Journal of virological methods}, year={1997}, volume={63 1-2}, pages={47-56} }