Detection of MMP activity in living cells by a genetically encoded surface-displayed FRET sensor.

@article{Yang2007DetectionOM,
  title={Detection of MMP activity in living cells by a genetically encoded surface-displayed FRET sensor.},
  author={Jie Yang and Zhihong Zhang and Juqiang Lin and Jinling Lu and Bi-Feng Liu and Shaoqun Zeng and Qingming Luo},
  journal={Biochimica et biophysica acta},
  year={2007},
  volume={1773 3},
  pages={400-7}
}
Matrix metalloproteinases (MMPs) are secretory endopeptidases. They have been associated with invasion by cancer-cell and metastasis. Previous studies have demonstrated that proteolytic activity could be detected using fluorescence resonance energy transfer (FRET) with mutants of GFP. To monitor MMP activity, we constructed vectors that encoded a MMP Substrate Site (MSS) between YFP and CFP. In vitro, YFP-MSS-CFP can be used to detect MMP activity and 1,10-phenathroline inhibition of MMP… CONTINUE READING
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