Detection of Listeria monocytogenes in food using a combined enrichment/real-time PCR method targeting the prfA gene.

@article{Rossmanith2006DetectionOL,
  title={Detection of Listeria monocytogenes in food using a combined enrichment/real-time PCR method targeting the prfA gene.},
  author={Peter Rossmanith and Martina Krassnig and Martin Wagner and Ingeborg Hein},
  journal={Research in microbiology},
  year={2006},
  volume={157 8},
  pages={763-71}
}
A combined enrichment/real-time PCR method for the detection of Listeria monocytogenes is presented. The method is based on a conventional PCR assay targeting the prfA gene, which has been validated and suggested as an international standard PCR method for identifying L. monocytogenes in food. This real-time PCR assay includes an internal amplification control. Inclusivity and exclusivity were 100% each when testing 100 L. monocytogenes isolates, 30 Listeria spp. isolates other than L… CONTINUE READING

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