Detection of Idiotypic Cross-reactions among Streptococcal Antisera from Related Rabbits


Idiotypic cross-reactions among antibodies to Group C streptococcal carbohydrate were studied using idiotypic antisera prepared in allotypically matched rabbits. Antibodies with idiotypic cross-specificity to one proband antibody were detected in 58% of the antisera from related rabbits, while approximately 1% of nonrelated rabbits produced antibody with this specificity. The cross-specificity was related to the group a (V(H)) allotype of 133 rabbits tested with only one exception. Studies utilizing antisera against a second proband antibody failed to detect antibodies with idiotypic cross-reactivity among the same group of related rabbits. This result emphasizes the variation in expression of idiotypic determinants of antibodies. It was further shown that the presence of anti-IgG's in the streptococcal antisera interfere with the detection of idiotypic cross-reactions. These anti-IgG's masked the presence of antibodies with idiotypic cross-specificity when inhibition of precipitation tests were used for their detection.

3 Figures and Tables


Citations per Year

387 Citations

Semantic Scholar estimates that this publication has 387 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Kindt1973DetectionOI, title={Detection of Idiotypic Cross-reactions among Streptococcal Antisera from Related Rabbits}, author={Thomas J. Kindt and Rochelle K. Seide and Viktor A. Bokisch and Richard M. Krause}, journal={The Journal of Experimental Medicine}, year={1973}, volume={138}, pages={522 - 537} }