Detection of Idiotypic Cross-reactions among Streptococcal Antisera from Related Rabbits

Abstract

Idiotypic cross-reactions among antibodies to Group C streptococcal carbohydrate were studied using idiotypic antisera prepared in allotypically matched rabbits. Antibodies with idiotypic cross-specificity to one proband antibody were detected in 58% of the antisera from related rabbits, while approximately 1% of nonrelated rabbits produced antibody with this specificity. The cross-specificity was related to the group a (V(H)) allotype of 133 rabbits tested with only one exception. Studies utilizing antisera against a second proband antibody failed to detect antibodies with idiotypic cross-reactivity among the same group of related rabbits. This result emphasizes the variation in expression of idiotypic determinants of antibodies. It was further shown that the presence of anti-IgG's in the streptococcal antisera interfere with the detection of idiotypic cross-reactions. These anti-IgG's masked the presence of antibodies with idiotypic cross-specificity when inhibition of precipitation tests were used for their detection.

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@article{Kindt1973DetectionOI, title={Detection of Idiotypic Cross-reactions among Streptococcal Antisera from Related Rabbits}, author={Thomas J. Kindt and Rochelle K. Seide and Viktor A. Bokisch and Richard M. Krause}, journal={The Journal of Experimental Medicine}, year={1973}, volume={138}, pages={522 - 537} }