Detection of Herpes simplex virus DNA by real-time PCR.

@article{Kessler2000DetectionOH,
  title={Detection of Herpes simplex virus DNA by real-time PCR.},
  author={Harald H. Kessler and Gerhard M{\"u}hlbauer and Beate Rinner and Evelyn Stelzl and Anja Berger and Hans Wilhelm Doerr and Brigitte I. Santner and Elisabeth Marth and Holger Felix Rabenau},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 7},
  pages={2638-42}
}
Molecular detection of herpes simplex virus (HSV) DNA is recognized as the reference standard assay method for the sensitive and specific diagnosis of central nervous system infections caused by HSV. In this study, a molecular assay based on real-time PCR on the LightCycler (LC) instrument was evaluated and compared with a home-brew molecular assay. The detection limit of the LC assay was determined with 10-fold dilutions of plasmid pS4 with the SalI restriction fragment of the DNA polymerase… CONTINUE READING
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Qualitative detection of herpes simplex virus DNA on the LightCycler

  • H. H. Kessler
  • 2000
2 Excerpts

Selection of hybridization probe sequences for use with the LightCycler

  • O. Landt
  • 2000
1 Excerpt

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