Detection of Haemophilus parasuis isolates from South China by loop-mediated isothermal amplification and isolate characterisation.

@article{Zhang2012DetectionOH,
  title={Detection of Haemophilus parasuis isolates from South China by loop-mediated isothermal amplification and isolate characterisation.},
  author={Jian-min Zhang and Hai-yan Shen and Ming Liao and Tao Ren and Li-li Guo and Cheng-gang Xu and Sai-Xiang Feng and H. Fan and Jing-yi Li and Ji-dang Chen and Bin Zhang},
  journal={The Onderstepoort journal of veterinary research},
  year={2012},
  volume={79 1},
  pages={
          E1-6
        }
}
Haemophilus parasuis is the etiological agent of Glässer's disease, which is characterised by fibrinous polyserositis, meningitis and polyarthritis, causing severe economic losses to the swine industry. In this study, a loop-mediated isothermal amplification (LAMP) test was developed to improve the specificity, facility and speed of diagnosis of H. parasuis isolates. The LAMP assay rapidly amplified the target gene within 50 min incubation at 63 °C in a laboratory water bath. The LAMP amplicon… CONTINUE READING

References

Publications referenced by this paper.
SHOWING 1-10 OF 27 REFERENCES

Validation of a real-time PCR for Haemophilus parasuis.

  • Journal of applied microbiology
  • 2010
VIEW 5 EXCERPTS
HIGHLY INFLUENTIAL

Development of a PCR test to diagnose Haemophilus parasuis infections.

  • Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
  • 2001
VIEW 5 EXCERPTS
HIGHLY INFLUENTIAL

Loop - mediated is othermal amplification targeting the apxIVA gene for detection of Actinobacillus pleuropneumoniae ’

Y. Wang, P. Chen, +3 authors Q. G. He
  • FEMS Microbiology Letters
  • 2009
VIEW 1 EXCERPT