Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal

@article{Dreyer2013DetectionOH,
  title={Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal},
  author={Johannes H. Dreyer and Franziska Hauck and Michelle Oliveira-Silva and M{\'a}rio Henrique Magalh{\~a}es Barros and Gerald Niedobitek},
  journal={Virchows Archiv},
  year={2013},
  volume={462},
  pages={381-389}
}
Detecting human papillomavirus (HPV) infection in head and neck squamous cell carcinoma (HNSCC) is clinically relevant, but there is no agreement about the most appropriate methodology. [] Key Method We have studied 64 oropharyngeal carcinomas using p16 immunohistochemistry, HPV DNA in situ hybridisation (ISH) and HPV DNA polymerase chain reaction (PCR) followed by pyrosequencing. We have also evaluated a new assay, RNAscope, designed to detect HPV E6/E7 RNA transcripts. Using a threshold of 70 % labelled…

The presence of high-risk human papillomavirus (HPV) E6/E7 mRNA transcripts in a subset of sinonasal carcinomas is evidence of involvement of HPV in its etiopathogenesis

It is confirmed that HR-HPV is actively involved in the etiopathogenesis of a significant subset of sinonasal SCCs, and p16 may be used as a reliable surrogate marker for determination of HPV status also in sinonal S CCs.

pRb and CyclinD1 Complement p16 as Immunohistochemical Surrogate Markers of HPV Infection in Head and Neck Cancer

Study of HNSCC cases with known p16 and HPV DNA polymerase chain reaction (PCR) status for expression of retinoblastoma protein (pRb) and CyclinD1 by immunohistochemistry concludes that HPV-positive H NSCC are characterized by p16 overexpression and low scores for pRb, Cyclin D1, and a low combined pRB/CyclinD 1 score.

High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study

It is shown that HPV DNA in pretherapeutic mouthwashes can detect HPV-driven HNSCCs with a sensitivity of 50.0% and specificity of 85.4%, alongside a high negative predictive value of 79.5% and an accuracy of 74.5%.

HPV , protein p 16 and squamous cell carcinoma of the oral cavity

The data suggest that there is no effect of HPV status on the prognosis of OSCC patients compared to other HNSCC locations and there was no correlation between p16 positivity relative to the time to progression.

Prevalence of HPV infection in head and neck carcinomas shows geographical variability: a comparative study from Brazil and Germany

Analysis of head and neck squamous cell carcinomas from the oral cavity, oropharynx and hypopharynx using p16 immunohistochemistry, HPV DNA PCR and sequencing, hrHPV DNA in situ hybridisation (ISH) and HRHPV E6/E7 RNA ISH suggests areas with high and low prevalences of HPV infection in OPSCC may be possible to define.

HPV, protein p16 and squamous cell carcinoma of the oral cavity.

The data suggest that there is no effect of HPV status on the prognosis of OSCC patients compared to other HNSCC locations and there was no correlation between p16 positivity and the time to progression.

Feasibility of Immunohistochemical p16 Staining in the Diagnosis of Human Papillomavirus Infection in Patients With Squamous Cell Carcinoma of the Head and Neck: A Systematic Review and Meta-Analysis

Subgroup analysis showed that the IHC-p16 test was more consistent with the in situ hybridization (ISH) test and has greater diagnostic value for oropharyngeal squamous cell carcinoma, suggesting that their combination can be used to improve the specificity of diagnosis.

[HPV-associated oropharyngeal carcinoma. Status quo and relationship with cancer of unknown primary].

In a situation of CUP diagnostic testing for HPV, in negative cases in addition to EBV is recommended in lymph node metastases, due to the high predictive value for the localization of occult primary carcinomas.

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