Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal

  title={Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal},
  author={Johannes H. Dreyer and Franziska Hauck and Michelle Oliveira-Silva and M{\'a}rio Henrique Magalh{\~a}es Barros and Gerald Niedobitek},
  journal={Virchows Archiv},
Detecting human papillomavirus (HPV) infection in head and neck squamous cell carcinoma (HNSCC) is clinically relevant, but there is no agreement about the most appropriate methodology. [] Key Method We have studied 64 oropharyngeal carcinomas using p16 immunohistochemistry, HPV DNA in situ hybridisation (ISH) and HPV DNA polymerase chain reaction (PCR) followed by pyrosequencing. We have also evaluated a new assay, RNAscope, designed to detect HPV E6/E7 RNA transcripts. Using a threshold of 70 % labelled…

The presence of high-risk human papillomavirus (HPV) E6/E7 mRNA transcripts in a subset of sinonasal carcinomas is evidence of involvement of HPV in its etiopathogenesis

It is confirmed that HR-HPV is actively involved in the etiopathogenesis of a significant subset of sinonasal SCCs, and p16 may be used as a reliable surrogate marker for determination of HPV status also in sinonal S CCs.

pRb and CyclinD1 Complement p16 as Immunohistochemical Surrogate Markers of HPV Infection in Head and Neck Cancer

Study of HNSCC cases with known p16 and HPV DNA polymerase chain reaction (PCR) status for expression of retinoblastoma protein (pRb) and CyclinD1 by immunohistochemistry concludes that HPV-positive H NSCC are characterized by p16 overexpression and low scores for pRb, Cyclin D1, and a low combined pRB/CyclinD 1 score.

High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study

It is shown that HPV DNA in pretherapeutic mouthwashes can detect HPV-driven HNSCCs with a sensitivity of 50.0% and specificity of 85.4%, alongside a high negative predictive value of 79.5% and an accuracy of 74.5%.

HPV , protein p 16 and squamous cell carcinoma of the oral cavity

The data suggest that there is no effect of HPV status on the prognosis of OSCC patients compared to other HNSCC locations and there was no correlation between p16 positivity relative to the time to progression.

Prevalence of HPV infection in head and neck carcinomas shows geographical variability: a comparative study from Brazil and Germany

Analysis of head and neck squamous cell carcinomas from the oral cavity, oropharynx and hypopharynx using p16 immunohistochemistry, HPV DNA PCR and sequencing, hrHPV DNA in situ hybridisation (ISH) and HRHPV E6/E7 RNA ISH suggests areas with high and low prevalences of HPV infection in OPSCC may be possible to define.

HPV, protein p16 and squamous cell carcinoma of the oral cavity.

The data suggest that there is no effect of HPV status on the prognosis of OSCC patients compared to other HNSCC locations and there was no correlation between p16 positivity and the time to progression.

Feasibility of Immunohistochemical p16 Staining in the Diagnosis of Human Papillomavirus Infection in Patients With Squamous Cell Carcinoma of the Head and Neck: A Systematic Review and Meta-Analysis

Subgroup analysis showed that the IHC-p16 test was more consistent with the in situ hybridization (ISH) test and has greater diagnostic value for oropharyngeal squamous cell carcinoma, suggesting that their combination can be used to improve the specificity of diagnosis.

[HPV-associated oropharyngeal carcinoma. Status quo and relationship with cancer of unknown primary].

In a situation of CUP diagnostic testing for HPV, in negative cases in addition to EBV is recommended in lymph node metastases, due to the high predictive value for the localization of occult primary carcinomas.



Evaluation of human papillomavirus testing for squamous cell carcinoma of the tonsil in clinical practice

Using a three-tiered, staged algorithm, in conjunction with consensus reporting of HPV ISH, leads to less equivocal molecular classification of oropharyngeal SCCs, a subtype of head-and-neck cancer with a distinct clinical and prognostic profile.

A comparison of clinically utilized human papillomavirus detection methods in head and neck cancer

Compared with a gold standard HPV-PCR assay, HPV detection by in situ hybridization was less accurate for head and neck squamous cell carcinoma on tissue microarrays than p16 immunohistochemistry and further testing is warranted before these assays should be recommended for clinical HPV detection.

A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen

It is shown that clinically meaningful viral HPV infections can be more reliably measured in FFPE H NSCC samples in a standard and high throughput manner, paving the way for prognostic and experimental vaccination studies, regarding not only HNSCC, but possibly also cancer types with HPV involvement in subgroups such as penile and anal cancer.

Evaluation of Human Papilloma Virus Diagnostic Testing in Oropharyngeal Squamous Cell Carcinoma: Sensitivity, Specificity, and Prognostic Discrimination

Caution must be exercised in applying HPV16 diagnostic tests because of significant disparities in accuracy and prognostic value in previously published techniques.

Human papillomavirus status in young patients with head and neck squamous cell carcinoma

The presence of p16ink4a accumulation and the absence of p53 overexpression are good surrogate markers for HPV‐associated HNSCC, and there was no association of HPV positivity with (lack of) exposure to alcohol and smoking.

Biomarkers of HPV in head and neck squamous cell carcinoma.

A stronger association of HPV presence with prognosis (assessed by all-cause survival) is observed when "HPV-associated" HNSCC is defined using tumor status (HPV DNA status or P16) and HPV E6/E7 serology in combination rather using tumor HPV status alone.

HPV DNA detection in tumours of the head and neck: a comparative light microscopy and DNA hybridization study.

A series of 22 squamous cell carcinomas of the oral cavity, (naso-)pharynx and larynx were studied by conventional light microscopy and filter (dot blot) hybridization for the detection of human papillomavirus (HPV) DNA, providing further evidence for the presence of HPV infections in tumours of the upper respiratory and digestive tract.

High-Risk Human Papillomavirus E6/E7 mRNA Detection by a Novel In Situ Hybridization Assay Strongly Correlates With p16 Expression and Patient Outcomes in Oropharyngeal Squamous Cell Carcinoma

RNA ISH is more sensitive than DNA ISH in detecting HPV in OSCC, and it correlates strongly with p16, although both tests were comparable, p16 more strongly stratified patient outcomes.

Validation of Methods for Oropharyngeal Cancer HPV Status Determination in US Cooperative Group Trials

The HPV classification methods used in US cooperative group trials are validated and p16 IHC and HPV16 ISH assays show excellent performance, with high sensitivity and specificity, respectively.

Expression of p16 protein identifies a distinct entity of tonsillar carcinomas associated with human papillomavirus.