Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal

@article{Dreyer2013DetectionOH,
  title={Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal},
  author={Johannes H. Dreyer and Franziska Hauck and Michelle Oliveira-Silva and M{\'a}rio Henrique Magalh{\~a}es Barros and Gerald Niedobitek},
  journal={Virchows Archiv},
  year={2013},
  volume={462},
  pages={381-389}
}
Detecting human papillomavirus (HPV) infection in head and neck squamous cell carcinoma (HNSCC) is clinically relevant, but there is no agreement about the most appropriate methodology. [...] Key Method We have studied 64 oropharyngeal carcinomas using p16 immunohistochemistry, HPV DNA in situ hybridisation (ISH) and HPV DNA polymerase chain reaction (PCR) followed by pyrosequencing. We have also evaluated a new assay, RNAscope, designed to detect HPV E6/E7 RNA transcripts. Using a threshold of 70 % labelled…Expand
Interpretation of HPV DNA in situ hybridization in HPV-related head and neck squamous cell carcinoma: an achievable task in cell block and small biopsy material.
TLDR
HPV-related head and neck squamous cell carcinoma cases are evaluated for a semi-quantitative assessment of the ease of interpretation, staining pattern, and highest magnification needed for HPV DNA in-situ hybridization (ISH) on cell block and core biopsy. Expand
Identification of transcriptionally active HPV infection in formalin-fixed, paraffin-embedded biopsies of oropharyngeal carcinoma.
TLDR
The high sensitivity and specificity of the established combination of p16 immunostain, DNA ISH, and HPV16 DNA amplification suggests that the introduction of labour- and cost-intensive mRNA ISH is not necessary in the diagnostic routine of oropharyngeal tumors. Expand
The presence of high-risk human papillomavirus (HPV) E6/E7 mRNA transcripts in a subset of sinonasal carcinomas is evidence of involvement of HPV in its etiopathogenesis
TLDR
It is confirmed that HR-HPV is actively involved in the etiopathogenesis of a significant subset of sinonasal SCCs, and p16 may be used as a reliable surrogate marker for determination of HPV status also in sinonal S CCs. Expand
pRb and CyclinD1 Complement p16 as Immunohistochemical Surrogate Markers of HPV Infection in Head and Neck Cancer
TLDR
Study of HNSCC cases with known p16 and HPV DNA polymerase chain reaction (PCR) status for expression of retinoblastoma protein (pRb) and CyclinD1 by immunohistochemistry concludes that HPV-positive H NSCC are characterized by p16 overexpression and low scores for pRb, Cyclin D1, and a low combined pRB/CyclinD 1 score. Expand
HPV , protein p 16 and squamous cell carcinoma of the oral cavity
Background. Squamous cell carcinoma of the oral cavity is generally caused by the long-term impact of known risk factors, e.g. tobacco and alcohol, along with chronic traumatisation. A number ofExpand
Prevalence of HPV infection in head and neck carcinomas shows geographical variability: a comparative study from Brazil and Germany
TLDR
Analysis of head and neck squamous cell carcinomas from the oral cavity, oropharynx and hypopharynx using p16 immunohistochemistry, HPV DNA PCR and sequencing, hrHPV DNA in situ hybridisation (ISH) and HRHPV E6/E7 RNA ISH suggests areas with high and low prevalences of HPV infection in OPSCC may be possible to define. Expand
HPV, protein p16 and squamous cell carcinoma of the oral cavity.
TLDR
The data suggest that there is no effect of HPV status on the prognosis of OSCC patients compared to other HNSCC locations and there was no correlation between p16 positivity and the time to progression. Expand
Feasibility of Immunohistochemical p16 Staining in the Diagnosis of Human Papillomavirus Infection in Patients With Squamous Cell Carcinoma of the Head and Neck: A Systematic Review and Meta-Analysis
TLDR
Subgroup analysis showed that the IHC-p16 test was more consistent with the in situ hybridization (ISH) test and has greater diagnostic value for oropharyngeal squamous cell carcinoma, suggesting that their combination can be used to improve the specificity of diagnosis. Expand
[HPV-associated oropharyngeal carcinoma. Status quo and relationship with cancer of unknown primary].
TLDR
In a situation of CUP diagnostic testing for HPV, in negative cases in addition to EBV is recommended in lymph node metastases, due to the high predictive value for the localization of occult primary carcinomas. Expand
p16INK4 expression is of prognostic and predictive value in oropharyngeal cancers independent of human papillomavirus status: a Hungarian study
Head and neck cancer treatment protocols still lack well-established biomarkers of prognostic and predictive value. It is well known that human papillomavirus (HPV)-related and non-HPV-relatedExpand
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