Detection of Flexibacter maritimus in fish tissue using nested PCR amplification.

@article{Cepeda2003DetectionOF,
  title={Detection of Flexibacter maritimus in fish tissue using nested PCR amplification.},
  author={C. Cepeda and S. Garcı́a-M{\'a}rquez and Y. Santos},
  journal={Journal of fish diseases},
  year={2003},
  volume={26 2},
  pages={
          65-70
        }
}
A nested polymerase chain reaction (PCR) system was developed for the detection of Flexibacter maritimus from fish tissue. The total procedure for the diagnosis of marine flexibacteriosis, from the point of DNA extraction to the electrophoretic analysis, can be performed in < 4 h. This was achieved by the combination of a short thermal cycling programme with a rapid DNA extraction procedure. The assay was extremely sensitive, capable of detecting as few as 75 cfu mg(-1) fish tissue. The… Expand
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Comparison of serological and molecular typing methods for epidemiological investigation of Tenacibaculum species pathogenic for fish
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