Detection of FRET efficiency in imaging systems by photo-bleaching acceptors.

@article{Deng2010DetectionOF,
  title={Detection of FRET efficiency in imaging systems by photo-bleaching acceptors.},
  author={Chuyun Deng and Jiamin Li and Wanyun Ma},
  journal={Talanta},
  year={2010},
  volume={82 2},
  pages={771-4}
}
Fluorescence resonance energy transfer (FRET) is widely used to obtain the distance between a donor and an acceptor in biological research. However, the detection of FRET efficiencies with fluorescence microscopy imaging systems remains a great challenge due to the difficulties of transferring gray scales of the images into fluorescence intensities, and the… CONTINUE READING