Detection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreak.

@article{Paddock2013DetectionOE,
  title={Detection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreak.},
  author={Zac D Paddock and Jiawei Bai and Xiaorong Shi and David G. Renter and T G Nagaraja},
  journal={Applied and environmental microbiology},
  year={2013},
  volume={79 11},
  pages={3522-5}
}
A multiplex PCR was designed to detect Escherichia coli O104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregative E. coli, in cattle feces. A total of 248 fecal samples were tested, and 20.6% were positive for serogroup O104. The O104 isolates did not carry genes characteristic of the virulent hybrid strain. 
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