Detection of Enterobacter sakazakii strains by real-time PCR.

  title={Detection of Enterobacter sakazakii strains by real-time PCR.},
  author={Burkhard Malorny and Martin Wagner},
  journal={Journal of food protection},
  volume={68 8},
A precise 5' nuclease (TaqMan) real-time PCR was developed and validated in house for the specific detection of Enterobacter sakazakii isolates. Specifically designed nonpatented primers and a hydrolysis (TaqMan) probe were used to target the 16S rRNA gene. All 27 E. sakazakii and 141 non-E. sakazakii strains tested with the real-time PCR were identified correctly. To monitor false-negative results, an internal amplification control was coamplified with the same primers used for the E… CONTINUE READING
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