Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction

@article{Nmeth2011DetectionOD,
  title={Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction},
  author={Vikt{\'o}ria N{\'e}meth and M{\'o}nika Madai and Alexandra Mar{\'a}czi and B{\'a}lint B{\'e}rczi and Győző Horv{\'a}th and Mikl{\'o}s Oldal and P{\'e}ter Kisfali and K B{\'a}nyai and Ferenc Jakab},
  journal={Archives of Virology},
  year={2011},
  volume={156},
  pages={1655-1660}
}
Dobrava (DOBV) hantaviruses belong to the genus Hantavirus, family Bunyaviridae, and are carried by yellow-necked and striped field mice. The goal of this study was to detect DOBV using serological and genetic methods in Apodemus rodents in Hungary and in northern Croatia. During the study period, a total of 125 Apodemus sp. (67 A. agrarius, 58 A. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rRT-PCR and/or ELISA. We conclude that the… CONTINUE READING

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