Detection of Dientamoeba fragilis in fresh stool specimens using PCR.

  title={Detection of Dientamoeba fragilis in fresh stool specimens using PCR.},
  author={Damien J Stark and Nigel W. Beebe and Deborah Marriott and John T. Ellis and Jock Harkness},
  journal={International journal for parasitology},
  volume={35 1},
Dientamoeba fragilis is a trichomonad parasite that causes human gastrointestinal disease. Currently microscopy is considered to be the gold standard for diagnosis of D. fragilis infection. However, this method is time-consuming and relatively insensitive. A PCR assay based on the small-subunit ribosomal RNA gene of D. fragilis for the specific detection of D. fragilis DNA in fresh unpreserved stool samples was developed. The D. fragilis PCR was positive in 29/31 samples with positive… CONTINUE READING

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