Detection of Deletion Mutants in Rice Via Overgo Hybridization Onto Membrane Spotted Arrays

@article{Diaz2007DetectionOD,
  title={Detection of Deletion Mutants in Rice Via Overgo Hybridization Onto Membrane Spotted Arrays},
  author={Marco Quevedo Diaz and Monika Ryba and Hei Leung and Rodney L. Nelson and Jan E Leach},
  journal={Plant Molecular Biology Reporter},
  year={2007},
  volume={25},
  pages={17-26}
}
Overlapping nucleotides or overgos were designed and used to detect deletions in rice mutants that had been generated by treatment with a chemical (diepoxybutane, DB) or irradiation (gamma ray, GR). As a proof of concept, DNA of three spotted leaf 11 (spl11) deletion mutants, GR5612, GR5717 and DB2487, and the wild-type DNA (IR64) were spotted onto nylon membranes in different concentrations and probed with radiolabeled overgos designed from different regions within the Spl11 gene. At least 3… CONTINUE READING

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