Detection of DNA damage in yolk-sac larvae of the Japanese Medaka, Oryzias latipes, by the comet assay.

@article{Morin2011DetectionOD,
  title={Detection of DNA damage in yolk-sac larvae of the Japanese Medaka, Oryzias latipes, by the comet assay.},
  author={B{\'e}n{\'e}dicte Morin and Julien Filatreau and Ludovic Vicquelin and Iris Barjhoux and Sylvain Guinel and Joelle Leray-Forget and J{\'e}r{\^o}me Cachot},
  journal={Analytical and bioanalytical chemistry},
  year={2011},
  volume={399 6},
  pages={2235-42}
}
This study was set up to determine the suitability of the early life stage (ELS) alkaline comet assay for the detection of DNA strand breaks induced by genotoxicants in whole organism. This assay was performed on cells of medaka 2 days posthatch (dph). An efficient procedure for cell dissociation using enzymatic and mechanical digestion was developed. This… CONTINUE READING