Detection of DNA damage in response to cooling injury in equine spermatozoa using single-cell gel electrophoresis.


Single-cell gel electrophoresis (SCGE), or comet assay, has the ability to detect damage at the single cell level and has not been reported for equine sperm. The ability to detect nuclear damage at the single cell level could aid in the advancement of protocols for optimal semen preservation. The goals of these experiments were to adapt this assay for use… (More)


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