Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids.

@article{Vollmer1997DetectionOD,
  title={Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids.},
  author={Amy Cheng Vollmer and Saveliy Belkin and Dana R. Smulski and Tina K Van Dyk and Robert A Larossa},
  journal={Applied and environmental microbiology},
  year={1997},
  volume={63 7},
  pages={2566-71}
}
Plasmids were constructed in which DNA damage-inducible promoters recA, uvrA, and alkA from Escherichia coli were fused to the Vibrio fischeri luxCDABE operon. Introduction of these plasmids into E. coli allowed the detection of a dose-dependent response to DNA-damaging agents, such as mitomycin and UV irradiation. Bioluminescence was measured in real time over extended periods. The fusion of the recA promoter to luxCDABE showed the most dramatic and sensitive responses. lexA dependence of the… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 67 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 55 references

Escherichia coli and Salmonella: cellular and molecular biology

G. C. Walker
The SOS response of Escherichia coli, • 1996
View 2 Excerpts

Induction of the heat shock response and the SOS response in Escherichia coli by the effects of acoustic cavitation from ultrasound

A. C. Vollmer, I.R.S. Maken, E. C. Everbach
abstr. I-85, • 1996
View 1 Excerpt

Induction of the heat shock response and the SOS response in Escherichia coli by the effects of acoustic cavitation from ultrasound , abstr . I - 85 , p . 317

E. C. Everbach
1996

Fusions of Vibrio fischeri lux genes to Escherichia coli stress promoters : detection of environmental stress

A. K. Campbell, L. J. Kricka, P. E. Stanley
Bioluminescence and chemiluminescence : fundamentals and applied aspects • 1995

Fusions of Vibrio fischeri lux genes to Escherichia coli stress promoters: detection of environmental stress, p. 147–150

T. K. Van Dyk, S. Belkin, +3 authors R. A. LaRossa
1995
View 1 Excerpt

Oxidative and DNA damaging agents induce luminescence in E. coli harboring lux fusions to stress promoters, p. 509–512

S. Belkin, A. C. Vollmer, +3 authors R. A. LaRossa
1995

Similar Papers

Loading similar papers…