Detection of DNA adducts originating from 1-methoxy-3-indolylmethyl glucosinolate using isotope-dilution UPLC-ESI-MS/MS.

@article{Schumacher2012DetectionOD,
  title={Detection of DNA adducts originating from 1-methoxy-3-indolylmethyl glucosinolate using isotope-dilution UPLC-ESI-MS/MS.},
  author={Fabian Schumacher and Wolfram Engst and Bernhard Hans Monien and Simone Florian and Anke Schnapper and Lisa Steinhauser and Klaus Albert and Heinz Frank and Albrecht Seidel and Hansruedi Glatt},
  journal={Analytical chemistry},
  year={2012},
  volume={84 14},
  pages={6256-62}
}
1-Methoxy-3-indolylmethyl (1-MIM) glucosinolate, present at substantial levels in several food crops (e.g., broccoli and cabbage), forms DNA adducts in vitro and is mutagenic to bacterial and mammalian cells after activation by the plant enzyme myrosinase. Moreover, a breakdown product, 1-MIM alcohol, is metabolized to a secondary reactive intermediate by some mammalian sulfotransferases (SULTs). First, we incubated herring-sperm DNA with 1-MIM glucosinolate in the presence of myrosinase. We… CONTINUE READING