Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods

@inproceedings{Pokorov2010DetectionOC,
  title={Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods},
  author={Dagmar Pokorov{\'a} and Stanislava Reschov{\'a} and Z. Nevor{\'a}nkov{\'a} and Monika Vicenova and Tom{\'a}{\vs} Vesel{\'y} and Veronika Pia{\vc}kov{\'a}},
  year={2010}
}
Cyprinid herpesvirus-3 (CyHV-3, previously described as koi herpesvirus) is a causative agent of the lethal disease in koi and common carp that can adversely affect common and koi carp breeds. Outbreaks with mass mortality have occurred world wide since 1998. At present, several different polymerase chain reaction (PCR) tests are in use to detect CyHV-3 in koi and common carp. In this study, we compared the sensitivity of the single-round PCR assays of Gray, Gilad, and Bercovier, and further… CONTINUE READING