Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure.

Abstract

The polymerase chain reaction (PCR) procedure was evaluated to see if it is a simple and rapid method to detect Clostridium perfringens enterotoxin gene. The method, involving the use of two synthesised primers and gene amplification by the PCR procedure, detects a DNA fragment of 364 base pairs of C. perfringens enterotoxin gene by gel electrophoresis. The… (More)

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Cite this paper

@article{Saito1992DetectionOC, title={Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure.}, author={Masahiro Saito and Masanori Matsumoto and Martha Funabashi}, journal={International journal of food microbiology}, year={1992}, volume={17 1}, pages={47-55} }