Detection of Clonal and Subclonal Copy-Number Variants in Cell-Free DNA from Patients with Breast Cancer Using a Massively Multiplexed PCR Methodology

Abstract

We demonstrate proof-of-concept for the use of massively multiplexed PCR and next-generation sequencing (mmPCR-NGS) to identify both clonal and subclonal copy-number variants (CNVs) in circulating tumor DNA. This is the first report of a targeted methodology for detection of CNVs in plasma. Using an in vitro model of cell-free DNA, we show that mmPCR-NGS… (More)
DOI: 10.1016/j.tranon.2015.08.004

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@inproceedings{Kirkizlar2015DetectionOC, title={Detection of Clonal and Subclonal Copy-Number Variants in Cell-Free DNA from Patients with Breast Cancer Using a Massively Multiplexed PCR Methodology}, author={Eser Kirkizlar and Bernhard Zimmermann and Tudor P Constantin and Ryan K Swenerton and Bin Hoang and Nicholas Wayham and Joshua E. Babiarz and Zachary P. Demko and Robert J. Pelham and Stephanie Kareht and Alexander Simon and Kristine N. Jinnett and Matthew Rabinowitz and Styrmir Sigurjonsson and Matthew Hill}, booktitle={Translational oncology}, year={2015} }