Detection of Citrus leprosis virus C using specific primers and TaqMan probe in one-step real-time reverse-transcription polymerase chain reaction assays.

@article{Choudhary2015DetectionOC,
  title={Detection of Citrus leprosis virus C using specific primers and TaqMan probe in one-step real-time reverse-transcription polymerase chain reaction assays.},
  author={Nandlal Choudhary and Gang W. Wei and Ananthakrishnan Govindarajulu and Avijit Roy and Wen-Bin Li and Deric D. Picton and Mark K. Nakhla and Laurene E Levy and Ronald H. Brlansky},
  journal={Journal of virological methods},
  year={2015},
  volume={224},
  pages={105-9}
}
Citrus leprosis virus C (CiLV-C), a causal agent of the leprosis disease in citrus, is mostly present in the South and Central America and spreading toward the North America. To enable better diagnosis and inhibit the further spread of this re-emerging virus a quantitative (q) real-time reverse transcription polymerase chain reaction (qRT-PCR) assay is needed for early detection of CiLV-C when the virus is present in low titer in citrus leprosis samples. Using the genomic sequence of CiLV-C… CONTINUE READING
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