Detection of Cells Translocated with Yersinia Yops in Infected Tissues Using β-Lactamase Fusions.

@article{Nguyen2019DetectionOC,
  title={Detection of Cells Translocated with Yersinia Yops in Infected Tissues Using $\beta$-Lactamase Fusions.},
  author={Giang T. Nguyen and Anne L. McCabe and Alyssa C. Fasciano and Joan Mecsas},
  journal={Methods in molecular biology},
  year={2019},
  volume={2010},
  pages={
          117-139
        }
}
Development of the TEM-CCF2/4-AM FRET-based system has enabled investigators to track translocation of effector proteins into mammalian cells during infection. This allows for separation of translocated and non-translocated cell populations for further study. Yersinia strains expressing translational Yop-TEM fusions, containing the secretion and translocation signals of a Yop with the TEM-1 portion of β-lactamase, are used to infect mice, tissues isolated from mice, or mammalian cells in… 
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