Detection of Candida and Aspergillus species DNA using broad-range real-time PCR for fungal endophthalmitis

@article{Sugita2011DetectionOC,
  title={Detection of Candida and Aspergillus species DNA using broad-range real-time PCR for fungal endophthalmitis},
  author={Sunao Sugita and Koju Kamoi and Masafumi Ogawa and Ken Watanabe and Norio Shimizu and Manabu Mochizuki},
  journal={Graefe's Archive for Clinical and Experimental Ophthalmology},
  year={2011},
  volume={250},
  pages={391-398}
}
The goal of this work is to establish a broad-range real-time polymerase chain reaction (PCR) diagnostic system for ocular fungal infection and to measure Candida and Aspergillus DNA in the ocular fluids obtained from unknown uveitis/endophthalmitis patients. After obtaining informed consent, intraocular fluids (aqueous humor and vitreous fluid samples) were collected from 54 patients with idiopathic uveitis or endophthalmitis. Samples were assayed for Candida or Aspergillus DNA using broad… CONTINUE READING
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