Detection of Biofilm in Wounds as an Early Indicator for Risk for Tissue Infection and Wound Chronicity

@article{Vyas2016DetectionOB,
  title={Detection of Biofilm in Wounds as an Early Indicator for Risk for Tissue Infection and Wound Chronicity},
  author={Krishna S. Vyas and Lesley K Wong},
  journal={Annals of Plastic Surgery},
  year={2016},
  volume={76},
  pages={127–131}
}
AbstractWounds are dynamic environments in which dead tissue, exudate, and the bacterial bioburden interact in a complex manner among themselves and with the tissue of the host. Bacteria organize into biofilms, reducing their susceptibility to elimination by the host immune response and antimicrobials. Early detection of biofilms in wounds is crucial to successful chronic wound management; and although many techniques have developed to identify planktonic counterparts in viable wounds, few have… 
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  • 2018
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TLDR
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TLDR
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TLDR
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...
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References

SHOWING 1-10 OF 72 REFERENCES
A review of the scientific evidence for biofilms in wounds
TLDR
The studies highlighted within this paper provide evidence that biofilms reside within the chronic wound and represent an important mechanism underlying the observed, delayed healing and infection.
Microscopic and physiologic evidence for biofilm‐associated wound colonization in vivo
TLDR
The results demonstrated that S. aureus form firmly attached microcolonies and colonies of bacteria encased in an extracellular matrix on the surface of the wounds, and these biofilm‐like communities demonstrated increased antimicrobial resistance when compared with their planktonic phenotype in vivo.
Towards diagnostic guidelines for biofilm-associated infections.
TLDR
This review is designed to give an overview of biofilm-associated infections (BAI) and to propose a platform for further discussion that includes clinicians, medical microbiologists, and biofilm researchers who are stakeholders in advancing the scientific pursuit of better diagnosis and treatment of BAI to mitigate their human and healthcare costs.
A Wound‐Isolated Pseudomonas aeruginosa Grows a Biofilm In Vitro Within 10 Hours and Is Visualized by Light Microscopy
TLDR
The rapidity of biofilm growth suggests that bacteria in wounds possess the capacity of producing this shield against antibiotics and immune effector cells early in the infection process, and efforts to prevent or slow the proliferation of bacteria and biofilms should occur soon after a wound is created.
Biofilm maturity studies indicate sharp debridement opens a time- dependent therapeutic window.
TLDR
The principles of biofilm-based wound care, along with the use of serial debridement to continually remove mature biofilm, followed by biofilm wound management strategies, including topical antibiotics while the bioburden is still immature and more susceptible, are valid.
Distribution, Organization, and Ecology of Bacteria in Chronic Wounds
TLDR
It is suggested that bacteria present within these wounds tend to be aggregated in microcolonies imbedded in a self-produced matrix, characteristic of the biofilm mode of growth, which supports the development of new diagnostic and treatment strategies for chronic wounds.
Bacterial biofilms: an emerging link to disease pathogenesis.
TLDR
This chapter discusses three infections that are caused by biofilms--infectious kidney stones, bacterial endocarditis, and cystic fibrosis lung infections--and focuses on the role of the biofilm in disease pathogenesis.
Evolving concepts in biofilm infections
TLDR
Several pathogens associated with chronic infections, including Pseudomonas aeruginosa in cystic fibrosis pneumonia, Haemophilus influenzae and Streptococcus pneumoniae in chronic otitis media, and enteropathogenic Escherichia coli in recurrent urinary tract infections, are linked to biofilm formation.
Development of a novel ex vivo porcine skin explant model for the assessment of mature bacterial biofilms
  • Qingping Yang, P. Phillips, G. Schultz
  • Biology, Medicine
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
  • 2013
TLDR
This ex vivo model simulates growth of biofilms on skin wounds and provides an accurate model to assess effects of antimicrobial agents on mature biofilmms.
Detection and quantification of bacterial biofilms combining high-frequency acoustic microscopy and targeted lipid microparticles
TLDR
It is demonstrated that acoustic impedance of biofilms is similar to the impedance of human tissues, making in vivo imaging and detection of biofilm matrices difficult, and the combination of ultrasound and targeted UCAs can be used to enhance biofilm imaging and early detection.
...
...