Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction.

@article{Nicolaiewsky2001DetectionOB,
  title={Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction.},
  author={T B Nicolaiewsky and Marc Richter and Vagner Ricardo Lunge and Cristina W Cunha and O Delagostin and Nilo Ikuta and Andr{\'e} Salvador Kazantzi Fonseca and S{\'e}rgio Silva da Silva and Luiz Shozo Ozaki},
  journal={Veterinary parasitology},
  year={2001},
  volume={101 1},
  pages={9-21}
}
We describe a nested polymerase chain reaction (PCR) for the detection of Babesia equi in equine infected erythrocytes using oligonucleotides designed on the published sequence of a B. equi merozoite antigen gene (ema-1). A 102bp DNA fragment is specifically amplified from B. equi but not from Babesia caballi, Babesia bovis or Babesia bigemina DNA. In a mock infection we were able to detect down to six infected cells in 10(8) equine erythrocytes or to detect the parasite in blood with an… CONTINUE READING

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