Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA.

@article{Fahrimal1992DetectionOB,
  title={Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA.},
  author={Y Fahrimal and Will L. Goff and Douglas P. Jasmer},
  journal={Journal of clinical microbiology},
  year={1992},
  volume={30 6},
  pages={
          1374-9
        }
}
Carrier cattle infected with Babesia bovis are difficult to detect because of the low numbers of parasites that occur in peripheral blood. However, diagnosis of low-level infections with the parasite is important for evaluating the efficacies of vaccines and in transmission and epidemiological studies. We used the polymerase chain reaction (PCR) to amplify a portion of the apocytochrome b gene from the parasite and tested the ability of this method to detect carrier cattle. The target sequence… CONTINUE READING

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