Detection of Australasian Flavivirus encephalitic viruses using rapid fluorogenic TaqMan RT-PCR assays.

@article{Pyke2004DetectionOA,
  title={Detection of Australasian Flavivirus encephalitic viruses using rapid fluorogenic TaqMan RT-PCR assays.},
  author={Alyssa T Pyke and Ina Smith and Andrew F van den Hurk and Judith A. Northill and Teck F Chuan and Alan J Westacott and Gregory A Smith},
  journal={Journal of virological methods},
  year={2004},
  volume={117 2},
  pages={161-7}
}
The development of single, sensitive, fluorogenic reverse transcriptase-polymerase chain reaction (TaqMan) assays were required for the rapid and specific detection of three encephalitic viruses found in the Australasian region, namely; Japanese encephalitis virus (JEV), Murray Valley encephalitis virus (MVEV), and Kunjin virus (KUNV). Primers and a fluorogenic probe were individually designed to be complementary to a nucleotide region encompassing the 3' terminus of the nonstructural (NS) 5… CONTINUE READING
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