Detection of African swine fever virus antibodies by immunoblotting assay.

  title={Detection of African swine fever virus antibodies by immunoblotting assay.},
  author={Mar{\'i}a Jos{\'e} Pastor and M D Laviada and Jos{\'e} M. S{\'a}nchez-Vizca{\'i}no and Jos{\'e} M. Escribano},
  journal={Canadian journal of veterinary research = Revue canadienne de recherche veterinaire},
  volume={53 1},
An immunoblotting assay has been adapted to detect antibodies against African swine fever virus. The electrophoretic transfer of proteins and the immunoreaction conditions were optimized, using 4 mA/cm2 of current intensity and 10 micrograms of soluble cytoplasmic antigen of infected cells per strip. Filters of polyvinylidene difluoride showed the highest capacity for protein absorption, but nitrocellulose filters showed lower backgrounds. The specificity and the pattern of the proteins induced… CONTINUE READING

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