Detection of Activity Responsible for Induction of the Agrobacterium tumefaciens Virulence Genes in Bacteriological Agar.

@article{Loubens1997DetectionOA,
  title={Detection of Activity Responsible for Induction of the Agrobacterium tumefaciens Virulence Genes in Bacteriological Agar.},
  author={Isabelle Loubens and William Scott Chilton and Patrick Dion},
  journal={Applied and environmental microbiology},
  year={1997},
  volume={63 11},
  pages={4578-80}
}
Agrobacterium tumefaciens C58 grown on acidic medium containing glucose and solidified with bacteriological agar expressed a virB::lacZ fusion. No expression of this fusion was observed on a similar medium which was solidified with purified agarose. The fraction from bacteriological agar which was responsible for vir gene induction was extracted with methanol and partially purified by preparative thin-layer chromatography. 

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