Detection of ALK gene rearrangements in formalin-fixed, paraffin-embedded tissue using a fluorescence in situ hybridization (FISH) probe: a search for optimum conditions of tissue archiving and preparation for FISH.

@article{Tan2003DetectionOA,
  title={Detection of ALK gene rearrangements in formalin-fixed, paraffin-embedded tissue using a fluorescence in situ hybridization (FISH) probe: a search for optimum conditions of tissue archiving and preparation for FISH.},
  author={Leonard Hwan Cheong Tan and Elaine Do and Siew Meng Chong and Evelyn Siew Chuan Koay},
  journal={Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology},
  year={2003},
  volume={7 1},
  pages={27-33}
}
BACKGROUND It is widely known that the efficiency of fluorescence in situ hybridization (FISH) probes applied to formalin-fixed, paraffin-embedded tissues is affected by the conditions under which the tissues are fixed and embedded. However, relatively few studies address exactly how tissue archiving conditions affect the performance of FISH probes. We report our experience based on use of an ALK FISH probe, during the validation of its diagnostic utility. METHODS We applied the probe to 77… CONTINUE READING