[Detection of ABO genotypes by simultaneous PCR-RFLP method].

  • Q Yang, C Zhu
  • Published 1999 in
    Zhonghua yi xue yi chuan xue za zhi = Zhonghua…


OBJECTIVE To study human ABO genotyping by means of multiplex PCR and restriction fragment length polymorphism. METHODS Two specific fragments of ABO gene were simultaneously amplified in a single tube, and then the double restriction digestion with RE Kpn I and Alu I was performed in the same tube. The amplified products were analyzed by PAGE and silver staining. A total of 125 Han unrelated individuals living in Wuhan were genotyped. RESULTS Six genotypes of ABO were detected and the distribution was in good agreement with Hardy-Weinberg equilibrium. CONCLUSION The results demonstrate that PCR-RFLP based approach is convenient, reliable and should be of value in forensic application.

Cite this paper

@article{Yang1999DetectionOA, title={[Detection of ABO genotypes by simultaneous PCR-RFLP method].}, author={Q Yang and C Zhu}, journal={Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics}, year={1999}, volume={16 2}, pages={110-2} }