Detection of 5-hydroxymethylcytosine in DNA by transferring a keto-glucose by using T4 phage β-glucosyltransferase.


5-Methylcytosine (5-mC) is the most important DNA modification found in eukaryotes. This base modification carries epigenetic information that regulates gene expression and disease pathogenesis. Recently, 5-hydroxymethylcytosine (5-hmC), another form of DNA modification generated by Tet dioxygenases, has been identified in substantial amounts in certain… (More)
DOI: 10.1002/cbic.201100278


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