Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine

Abstract

Various detection methods of the specific product of reaction of superoxide (O2•−) with hydroethidine (HE), namely 2-hydroxyethidium (2-OH-E+), and with its mitochondria-targeted analog are described. The detailed protocol for quantification of 2-OH-E+, the unique product of HE/O2•− in cellular systems, is presented. The procedure includes cell lysis… (More)
DOI: 10.1038/nprot.2007.473

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