Detection of 10 variants of biliverdin reductase in rat liver by two-dimensional gel electrophoresis.

@article{Huang1989DetectionO1,
  title={Detection of 10 variants of biliverdin reductase in rat liver by two-dimensional gel electrophoresis.},
  author={Tsung J Huang and G Michael Trakshel and M D Maines},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 14},
  pages={7844-9}
}
We have identified and characterized multiple forms of biliverdin reductase (BVR) in control rat liver cytosol. Two-dimensional electrophoresis of the purified BVR resolved a minimum of 10 discrete protein zones. All 10 proteins were BVR as judged by immunological cross-reactivity toward rabbit anti-rat BVR. Based on the isoelectric focusing pattern of separation, the BVR variants could be organized into five net-charge groups designated as BVR-IEF1 to BVR-IEF5 and three molecular mass groups… CONTINUE READING
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