Detection of 1,2-hydride shifts in the formation of euph-7-ene by the squalene-tetrahymanol cyclase of Tetrahymena pyriformis.

@article{Giner2005DetectionO1,
  title={Detection of 1,2-hydride shifts in the formation of euph-7-ene by the squalene-tetrahymanol cyclase of Tetrahymena pyriformis.},
  author={Jos{\'e}-Luis Giner and Stefano Rocchetti and Serge J.M. Neunlist and Michel. Rohmer and Duilio Arigoni},
  journal={Chemical communications},
  year={2005},
  volume={24},
  pages={
          3089-91
        }
}
Incubation of samples of 2,3-dihydrosqualene, specifically labeled with deuterium at either carbon position 7 or 11, with an enzyme extract from Tetrahymena pyriformis, containing a squalene-tetrahymanol cyclase, provided specimens of euph-7-enes displaying deuterium patterns consistent with the biosynthetic operation of two consecutive 1,2-hydride shifts. 
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TLDR
The cyclization of hexaprenyl methyl ether is the first attempt of identification of these tricyclopolyprenol derivatives in living organisms.
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