Detection of β-glucosidase as saprotrophic ability from an ectomycorrhizal mushroom, Tricholoma matsutake

@article{Kusuda2006DetectionO,
  title={Detection of $\beta$-glucosidase as saprotrophic ability from an ectomycorrhizal mushroom, Tricholoma matsutake},
  author={Mizuho Kusuda and Mitsuhiro Ueda and Yasuhito Konishi and Y Araki and Katsuji Yamanaka and Masami Nakazawa and Kazutaka Miyatake and Takao Terashita},
  journal={Mycoscience},
  year={2006},
  volume={47},
  pages={184-189}
}
We investigated extracellular carbohydrase production in the medium of an ectomycorrhizal fungus, Tricholoma matsutake, to reveal its ability to utilize carbohydrates such as starch as a growth substrate and to survey the saprotrophic aspects. We found β-glucosidase activity in the static culture filtrate of this fungus. The β-glucosidase was purified and characterized. The purified enzyme was obtained from about 2.1 l static culture filtrate, with 9.0% recovery, and showed a single protein… Expand
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TLDR
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References

SHOWING 1-10 OF 18 REFERENCES
Characterization of extracellular glucoamylase from the ectomycorrhizal mushroom Lyophyllum shimeji
TLDR
It is concluded that the amylase from L. shimeji seems to be a glucoamylase, which readily hydrolyzed the α-1,4 glucosidic linkage such as dextrin and amylose A, converting into glucose, and hydrolyze the cyclic polysaccharides. Expand
Purification and some properties of Α-amylase from an ectomycorrhizal fungus, Tricholoma matsutake
TLDR
Measurement of viscosity and TLC and HPLC analysis of the hydrolysates obtained from amylose showed that the amylase from T. matsutake is an endo-type (Α-amylase). Expand
Purification and characterisation of a beta-glucosidase (cellobiase) from a mushroom Termitomyces clypeatus.
TLDR
A beta-glucosidase with cellobiase activity was purified to homogeneity from the culture filtrate of the mushroom Termtomyces clypeatus and high molecular weight enzyme protein was present both intracellularly and extracellularly from the very early growth phase. Expand
Purification and Properties of α-Glucosidase and Glucoamylase from Lentinus edodes (Berk.) Sing.
An α-glucosidase and a glucoamylase have been isolated from fruit bodies of Lentinus edodes (Berk.) Sing., by a procedure including fractionation with ammonium sulfate, DEAE-cellulose columnExpand
Detection of the ability of Tricholoma matsutake to utilize sawdust in aseptic culture
TLDR
Four isolates of Tricholoma matsutake were investigated for growth on media supplemented with combinations of glucose and sawdust and it was suggested that isolate T945 of T. matsutakes is capable of utilizing sawdust in aseptic culture. Expand
Productivity of hydrolytic enzymes by mycorrhizal mushrooms
To survey the potential for production of extracellular hydrolytic enzymes by mycorrhizal mushrooms, productivities of these exo-enzymes from mycelia on potato-dextrose liquid medium wereExpand
The Extracellular Enzyme Activities in Culture Broth of Tricholoma matsutake
TLDR
The mycelia of Tricholoma matsutake DGUM 26001, 26101, 26210 and FRI 91024 were used and the specific activity of xylanase was comparatively high, however, little or no enzyme activities were found for , lignin enzyme, CMCase, chitinase, protease, and lipase. Expand
Ability of ectomycorrhizal fungi to utilize starch and related substrates
Basidiomycetous fungi of 55 strains of 33 species in 15 genera which are thought to be ectomycorrhizal were grown on starch and related substrates as a sole carbon source, and their ability toExpand
Saprobic potential of Tricholoma matsutake: growth over pine bark treated with surfactants
TLDR
Saprotrophic growth of Tricholoma matsutake isolates was investigated over Pinus densiflora bark fragments either on soil or on agar media, suggesting that Matsutake was able to extract some nutrients to sustain its growth. Expand
Enhancement of the viscometric endocellulase activity of Polyporus arcularius CMCase IIIa by cellobiose and cellooligosaccharides
TLDR
Inhibition of CMCases I and II by cellobiose and cellooligosaccharides may be the result of competition between the substrate and the reaction products, thus enhancing CMCase IIIa activity. Expand
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