Detection of β cell death in diabetes using differentially methylated circulating DNA.

@article{Akirav2011DetectionO,
  title={Detection of β cell death in diabetes using differentially methylated circulating DNA.},
  author={Eitan Moshe Akirav and Jasmin Lebastchi and Eva Galvan and Octavian Henegariu and Michael Akirav and Vitaly Ablamunits and Paul Lizardi and Kevan C Herold},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2011},
  volume={108 47},
  pages={
          19018-23
        }
}
In diabetes mellitus, β cell destruction is largely silent and can be detected only after significant loss of insulin secretion capacity. We have developed a method for detecting β cell death in vivo by amplifying and measuring the proportion of insulin 1 DNA from β cells in the serum. By using primers that are specific for DNA methylation patterns in β cells, we have detected circulating copies of β cell-derived demethylated DNA in serum of mice by quantitative PCR. Accordingly, we have… CONTINUE READING

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