Detection of "apoptosis-like" changes during the cryopreservation process in equine sperm.

  title={Detection of "apoptosis-like" changes during the cryopreservation process in equine sperm.},
  author={Cristina Ortega-Ferrusola and Y Sotillo-Gal{\'a}n and E Varela-Fern{\'a}ndez and Juan M Gallardo-Bola{\~n}os and Alfonso Muriel and L Gonz{\'a}lez-Fern{\'a}ndez and Jos{\'e} Antonio Rivera Tapia and F J Pe{\~n}a},
  journal={Journal of andrology},
  volume={29 2},
The kinematics of the appearance of apoptotic markers was studied by flow cytometry and immunoblot assays in equine spermatozoa subjected to freezing and thawing. Caspase activity, low mitochondrial membrane potential, and increases in sperm membrane permeability were observed in all of the phases of the cryopreservation procedure. Freezing and thawing caused an increase in membrane permeability and changes in the pattern of caspase activity; decreases in mitochondrial membrane potential were… CONTINUE READING


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