Detection by RT-PCR and genetic characterization of canine distemper virus from vaccinated and non-vaccinated dogs in Argentina.

@article{Calderon2007DetectionBR,
  title={Detection by RT-PCR and genetic characterization of canine distemper virus from vaccinated and non-vaccinated dogs in Argentina.},
  author={Marina Beatriz Gallo Calderon and Patricia Remorini and Osvaldo H Periolo and Marcela Iglesias and Nora Marta Mattion and Jos{\'e} Leonardo La Torre},
  journal={Veterinary microbiology},
  year={2007},
  volume={125 3-4},
  pages={
          341-9
        }
}
RT-PCR was used to detect canine distemper virus (CDV) RNA in clotted blood from Argentine domestic dogs. The NP gene was detected in 73 out of 99 blood samples analyzed. The deduced amino acid sequence of these gene fragments showed 100% identity with the sequence of other wild-type and vaccine strains. A fragment of the hemagglutinin gene was amplified from 24 (32.9%) of the NP-RNA-positive clinical specimens. These H fragments were further analyzed by restriction fragment length polymorphism… CONTINUE READING
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