Detection and structural investigation of metabolites of stanozolol in human urine by liquid chromatography tandem mass spectrometry

@article{Pozo2009DetectionAS,
  title={Detection and structural investigation of metabolites of stanozolol in human urine by liquid chromatography tandem mass spectrometry},
  author={{\'O}scar J. Pozo and Peter Van Eenoo and K Deventer and Leen Lootens and Susana Grimalt and Juan Vicente Sancho and F{\'e}lix Hern{\'a}ndez and Philip Meuleman and Geert Leroux-Roels and Frans T. Delbeke},
  journal={Steroids},
  year={2009},
  volume={74},
  pages={837-852}
}
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Using complementary mass spectrometric approaches for the determination of methylprednisolone metabolites in human urine.
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Expanding analytical possibilities concerning the detection of stanozolol misuse by means of high resolution/high accuracy mass spectrometric detection of stanozolol glucuronides in human sports drug testing.
TLDR
The commercial availability of 3'-OH-stanozolol glucuronide has been of great value for confirmatory purposes, and 17-epistanozolol-N-glucuronide was found to be a favourable long-term metabolite for doping controls as it was observed up to 28 days post-administration of the drug.
Sensitive and robust method for anabolic agents in human urine by gas chromatography-triple quadrupole mass spectrometry.
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References

SHOWING 1-10 OF 29 REFERENCES
Detection of Stanozolol and Its Major Metabolites in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry
The determination of stanozolol and its metabolites in human urine has been of particular interest in sports drug testing due to its frequently revealed misuse. A simple and rapid sample preparation
Development and validation of a qualitative screening method for the detection of exogenous anabolic steroids in urine by liquid chromatography-tandem mass spectrometry
TLDR
A screening method for the urinary detection of 34 exogenous anabolic steroids has been developed and limits of detection between 1 and 10 ng/ml were obtained for most analytes which fulfil current requirements.
High-performance liquid chromatography/tandem mass spectrometry: its use for the identification of stanozolol and its major metabolites in human and equine urine.
A screening procedure for the anabolic steroid stanozolol in human and equine urine was developed based on enzymatic hydrolysis, liquid-liquid extraction and reversed-phase liquid chromatography
Efficient approach for the comprehensive detection of unknown anabolic steroids and metabolites in human urine by liquid chromatography-electrospray-tandem mass spectrometry.
TLDR
An approach based on precursor ion scanning for the detection of unknown anabolic steroids and metabolites is proposed and only compounds with a steroidal structure showed a signal at all three selected m/z values although some exceptions have been found.
Elucidation of urinary metabolites of fluoxymesterone by liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry.
TLDR
A tentative elucidation as well as a proposal for the metabolic pathway of fluoxymesterone has been suggested using the ionization, the CID fragmentation, the accurate mass of the product ions and the EI spectra of these analytes.
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