Detection and structural investigation of metabolites of stanozolol in human urine by liquid chromatography tandem mass spectrometry

  title={Detection and structural investigation of metabolites of stanozolol in human urine by liquid chromatography tandem mass spectrometry},
  author={{\'O}scar J. Pozo and Peter Van Eenoo and K Deventer and Leen Lootens and Susana Grimalt and Juan Vicente Sancho and F{\'e}lix Hern{\'a}ndez and Philip Meuleman and Geert Leroux-Roels and Frans T. Delbeke},
Detection of mono-hydroxylated metabolites of stanozolol by HPLC-ESI (+) MS/MS in Indian sports persons.
It can be concluded that the marked increase in percent positive of stanozolol in Indian sportspersons in 2009 may be due to the improved detection by a more effective LCMS/MS method.
Combination of liquid-chromatography tandem mass spectrometry in different scan modes with human and chimeric mouse urine for the study of steroid metabolism.
A liquid chromatography tandem mass spectometry method based on a precursor ion scan with a uPA-SCID mouse with humanized liver (a chimeric mouse) was explored for the detection of steroid metabolism and three hitherto unreported metabolites were found.
Detection of stanozolol O- and N-sulfate metabolites and their evaluation as additional markers in doping control.
Results showed that sulfates do not improve the retrospectivity of the detection compared to the previously described long-term metabolite (epistanozolol-N-glucuronide), however, sulfate metabolites could be additional markers for the detection of STAN misuse.
P Detection and identification of glucuronidated stanozolol metabolites by liquid chromatography – tandem mass spectrometry
A study of the detection of glucuronidated stanozolol metabolites including 4β-hydroxystanozlol glucuronide (4STANG) and 16β-HydroxystANZolol glucuride (16STANG), which is reported to complete the development of an analytical method for the Detection of hydroxystanoZoll glucuronides metabolites.
Detection and characterization of urinary metabolites of boldione by LC-MS/MS. Part II: Conjugates with cysteine and N-acetylcysteine.
Up to the authors' knowledge this is the first time that cysteine conjugates are presented for exogenous anabolic androgenic steroids and the first report of N-acetylcysteine Conjugates for steroids.
Using complementary mass spectrometric approaches for the determination of methylprednisolone metabolites in human urine.
New metabolites have been identified in urine samples collected after oral administration of 40 mg of methylprednisolone and it is proposed that hydroxylation of the 6α-methyl group can take place.
Expanding analytical possibilities concerning the detection of stanozolol misuse by means of high resolution/high accuracy mass spectrometric detection of stanozolol glucuronides in human sports drug testing.
The commercial availability of 3'-OH-stanozolol glucuronide has been of great value for confirmatory purposes, and 17-epistanozolol-N-glucuronide was found to be a favourable long-term metabolite for doping controls as it was observed up to 28 days post-administration of the drug.
Sensitive and robust method for anabolic agents in human urine by gas chromatography-triple quadrupole mass spectrometry.


Detection of Stanozolol and Its Major Metabolites in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry
The determination of stanozolol and its metabolites in human urine has been of particular interest in sports drug testing due to its frequently revealed misuse. A simple and rapid sample preparation
Development and validation of a qualitative screening method for the detection of exogenous anabolic steroids in urine by liquid chromatography-tandem mass spectrometry
A screening method for the urinary detection of 34 exogenous anabolic steroids has been developed and limits of detection between 1 and 10 ng/ml were obtained for most analytes which fulfil current requirements.
High-performance liquid chromatography/tandem mass spectrometry: its use for the identification of stanozolol and its major metabolites in human and equine urine.
A screening procedure for the anabolic steroid stanozolol in human and equine urine was developed based on enzymatic hydrolysis, liquid-liquid extraction and reversed-phase liquid chromatography
Efficient approach for the comprehensive detection of unknown anabolic steroids and metabolites in human urine by liquid chromatography-electrospray-tandem mass spectrometry.
An approach based on precursor ion scanning for the detection of unknown anabolic steroids and metabolites is proposed and only compounds with a steroidal structure showed a signal at all three selected m/z values although some exceptions have been found.
Elucidation of urinary metabolites of fluoxymesterone by liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry.
A tentative elucidation as well as a proposal for the metabolic pathway of fluoxymesterone has been suggested using the ionization, the CID fragmentation, the accurate mass of the product ions and the EI spectra of these analytes.