Detection and quantitation of mutant K-ras codon 12 restriction fragments by capillary electrophoresis.

@article{Mitchell1995DetectionAQ,
  title={Detection and quantitation of mutant K-ras codon 12 restriction fragments by capillary electrophoresis.},
  author={Charles E. Mitchell and Steven Alan Belinsky and John F. Lechner},
  journal={Analytical biochemistry},
  year={1995},
  volume={224 1},
  pages={148-53}
}
Polymerase chain reaction amplification and BstNI endonuclease digestion were performed on DNA isolated from cell lines that were either homozygous (SW480, A549) or heterozygous (Calu 1, SK-LU-1, A427) for K-ras codon 12 mutations. Polyacrylamide gel electrophoresis showed that both mutant and wildtype (WT) bands were present in Calu-1, SK-LU-1, and A427 cell DNA; only the mutant bands were observed with SW480 and A549 DNA. The percentages of mutant and WT fragments were measured using… CONTINUE READING
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