Detection and quantitation of genetically modified maize (Bt-176 transgenic maize) by applying ligation detection reaction and universal array technology.

@article{Bordoni2004DetectionAQ,
  title={Detection and quantitation of genetically modified maize (Bt-176 transgenic maize) by applying ligation detection reaction and universal array technology.},
  author={Roberta Bordoni and Alessandra Mezzelani and Clarissa Consolandi and Andrea Frosini and Ermanno Rizzi and Bianca M. Castiglioni and Claudia Salati and Nelson Marmiroli and Rosangela Marchelli and Luigi Rossi Bernardi and Cristina Battaglia and Gianluca De Bellis},
  journal={Journal of agricultural and food chemistry},
  year={2004},
  volume={52 5},
  pages={1049-54}
}
We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was unequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of… CONTINUE READING