Detection and quantification of vitamin K(1) quinol in leaf tissues.

  title={Detection and quantification of vitamin K(1) quinol in leaf tissues.},
  author={Chlo{\"e} van Oostende and Joshua R Widhalm and Gilles J. C. Basset},
  volume={69 13},
Phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone; vitamin K(1)) is vital to plants. It is responsible for the one-electron transfer at the A(1) site of photosystem I, a process that involves turnover between the quinone and semi-quinone forms of phylloquinone. Using HPLC coupled with fluorometric detection to analyze Arabidopsis leaf extracts, we detected a third redox form of phylloquinone corresponding to its fully reduced - quinol-naphthoquinone ring (PhQH(2)). A method was developed to… CONTINUE READING
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Rochaix, Loss of phylloquinone in Chamydomonas affects plastoquinone pool size and photosystem II synthesis

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