Detection and quantification of the red tide dinoflagellate Karenia brevis by real-time nucleic acid sequence-based amplification.

@article{Casper2004DetectionAQ,
  title={Detection and quantification of the red tide dinoflagellate Karenia brevis by real-time nucleic acid sequence-based amplification.},
  author={Erica T. Casper and J H Paul and Matthew C Smith and Michael A. Gray},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 8},
  pages={
          4727-32
        }
}
Nucleic acid sequence-based amplification (NASBA) is an isothermal method of RNA amplification that has been previously used in clinical diagnostic testing. A real-time NASBA assay has been developed for the detection of rbcL mRNA from the red tide dinoflagellate Karenia brevis. This assay is sensitive to one K. brevis cell and 1.0 fg of in vitro transcript, with occasional detection of lower concentrations of transcript. The assay did not detect rbcL mRNA from a wide range of nontarget… CONTINUE READING

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