Detection and quantification of protein residues in food grade amino acids and nucleic acids using a dot-blot fluorescent staining method.

@article{Yamada2004DetectionAQ,
  title={Detection and quantification of protein residues in food grade amino acids and nucleic acids using a dot-blot fluorescent staining method.},
  author={Naoyuki Yamada and Shin-ichi Ozawa and Naoko Kageyama and Hiroshi Miyano},
  journal={Journal of agricultural and food chemistry},
  year={2004},
  volume={52 17},
  pages={
          5329-33
        }
}
Food allergies represent an important health problem in industrialized countries, such that detection and quantitative analysis of the protein considered to be the main allergen is crucial. A dot-blot fluorescent staining method for the detection and quantitative analysis of protein residues in food grade amino acids and nucleic acids is presented. This method combines fluorescence staining with dot-blotting onto PVDF membrane. Several standard proteins, such as bovine serum albumin (66 kDa… CONTINUE READING