Detection and quantification of genotoxicity in wastewater-treated Tetrahymena thermophila using the comet assay.

@article{Lah2004DetectionAQ,
  title={Detection and quantification of genotoxicity in wastewater-treated Tetrahymena thermophila using the comet assay.},
  author={Barbara Lah and S. Malovrh and Mojca Narat and Tadej {\vC}epeljnik and Romana Marin{\vs}ek-Logar},
  journal={Environmental toxicology},
  year={2004},
  volume={19 6},
  pages={545-53}
}
In the present study, the comet, or single-cell, gel electrophoresis assay was adapted for use with the ubiquitous unicellular protozoan Tetrahymena thermophila, and the method was evaluated for its ability to detect DNA damage induced by known genotoxins and wastewater samples. The original comet assay protocol was substantially modified (e.g., lower concentrations of detergents were used in the lysis buffer; electrophoresis time was reduced). Using the modified method, T. thermophila were… CONTINUE READING
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